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Formation of hydrogen peroxide was determined after addition to the solution of 10 l of 2-7dichlorofluorescein diacetate (2m in ethanol) and DCF fluorescence measured as described previously (Garca-Ruiz et al, 1995, 1997)

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In conclusion, Phase II enzymes are an interesting research field since they play an essential role in the metabolism of hundreds of foreign compounds as well as in regulation of metabolism and disposition of various endogenous biologically active substances and thus maintaining homeostasis in the human body

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